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KMID : 0620920090410040269
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Experimental & Molecular Medicine
2009 Volume.41 No. 4 p.269 ~ p.276
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Calcium overload is essential for the acceleration of staurosporine-induced cell death following neuronal differentiation in PC12 cells
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Seo Su-Ryeon
Seo Jeong-Taeg
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Abstract
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Differentiation of neuronal cells has been shown to accelerate stress-induced cell death, but the underlying mechanisms are not completely understood. Here, we find that early and sustained increase in cytosolic ([Ca2+]c) and mitochondrial Ca2+ levels ([Ca2+]m) is essential for the increased sensitivity to staurosporineinduced cell death following neuronal differentiation in PC12 cells. Consistently, pretreatment of differentiated PC12 cells with the intracellular Ca2+-chelator EGTA-AM diminished staurosporine-induced PARP cleavage and cell death. Furthermore, Ca2+ overload and enhanced vulnerability to staurosporine in differentiated cells were prevented by Bcl-XL overexpression. Our data reveal a new regulatory role for differentiation- dependent alteration of Ca2+ signaling in cell death in response to staurosporine.
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KEYWORD
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bcl-X protein, calcium, cell death, cell differentiation, PC12 cells, staurosporine
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